Journal:

Article Title: EXPANSION OF HIGHLY CYTOTOXIC HUMAN NATURAL KILLER CELLS FOR CANCER CELL THERAPY

doi: 10.1158/0008-5472.CAN-08-3712

Figure Lengend Snippet: (A) Schema of the NKAES method. Irradiated K562-mb15-41BBL cells are mixed with peripheral blood mononuclear cells (PBMC) at a 1 : 1.5 ratio. (B) NKAES-NK cell expansion from mononuclear cells from 50 healthy donors. Fold recovery of CD56+CD3- cells after 7 days of culture relative to the number of input cells is shown; bar: median. (C) Ki67 and CD25 expression were examined after 5 days of NKAES culture, a stage at which cultures typically still contain a majority of non-NK cells as well as NK cells in their initial phase of expansion; >90% CD56+ cells expressed both markers, whereas CD56- cells remained mostly Ki67- and CD25-.

Article Snippet: Cells The AML cell lines K562, HL-60, KG1 and U937 (American Type Culture Collection; Rockville, MD) were maintained in RPMI-1640 (Gibco, Grand Island, NY) with 10% fetal bovine serum (FBS; BioWhittaker, Walkersville, MD).

Techniques: Irradiation, Expressing

Journal:

Article Title: EXPANSION OF HIGHLY CYTOTOXIC HUMAN NATURAL KILLER CELLS FOR CANCER CELL THERAPY

doi: 10.1158/0008-5472.CAN-08-3712

Figure Lengend Snippet: NOD/scid-IL2RGnull mice (n = 6) were injected with K562 cells expressing luciferase (2×105) i.p. Then, NKAES-NK cells (1×107) from the same donor were injected every 2 days i.p in 3 mice, from day 1 to day 11 after K562 injection. All 6 mice received IL-2 25000 IU i.p. daily for 3 weeks. (A) Leukemia cell growth was visualized through luciferin injection and Xenogen imaging (ventral is shown). Leukemia progressed in all 3 mice not treated with NK cells (top panels) and euthanized between day 37 and 44. By contrast, leukemia progression was not apparent in 2 of the 3 mice receiving NK cells which remain alive and leukemia-free 8 months after the beginning of the experiment; in a third mouse, leukemia became detectable on day 43 (the mouse was euthanized on day 70; bottom panels). (B) Signal intensities (photons/second) detected in control (left) and NK-treated mice (right).

Article Snippet: Cells The AML cell lines K562, HL-60, KG1 and U937 (American Type Culture Collection; Rockville, MD) were maintained in RPMI-1640 (Gibco, Grand Island, NY) with 10% fetal bovine serum (FBS; BioWhittaker, Walkersville, MD).

Techniques: Injection, Expressing, Luciferase, Imaging, Control

Journal: OncoTargets and therapy

Article Title: Hypermethylation of secreted frizzled-related proteins predicts poor prognosis in non-M3 acute myeloid leukemia

doi: 10.2147/OTT.S136502

Figure Lengend Snippet: Correlation between SFRP1 hypermethylation and expression level in non-M3 AML patients and HL60 cell line (since the value was lg function conversion, methylation and expression level at zero was excluded). Abbreviation: AML, acute myeloid leukemia.

Article Snippet: Seven human AML cell lines (SHI-1, THP-1, U937, HEL, HL60, K562, and NB4) (ATCC, Manassas, VA, USA) were routinely cultured in IMDM with 10% fetal bovine serum (ExCell Bio, Shanghai, People’s Republic of China) and grown at 37°C in 5% CO 2 humidified atmosphere.

Techniques: Expressing, Methylation